活力苏口服液对自然衰老小鼠肝、脑、肾组织p16基因表达的影响

摘要

Objective It is to observe the effect of Huolisu oral liquid on pl6 gene expression in liver, brain, renal tissue of aging mice, and to explore its mechanism of antiaging. Methods Aging mice were chosen and treated with Huolisu oral liquid, then the expression of mRNA of pl6 gene in liver, brain, renal tissue of aging mice was observed with real time fluorescent quantitative PCR. Results mRNA expression of p16 gene in liver, brain, renal tissue of aging mice in every different dosage of Huolisu oral liquid group were decreased, compared with that in the control group of aging model, the differences were all significant( P <0.05 ). Conclusion Huolisu oral liquid can decrease the expression of p16 gene in liver, brain and renal tissue in aging mice, thus to play an antiaging effect.%目的 观察活力苏口服液对自然衰老小鼠肝、脑、肾组织p16基因的影响,探讨活力苏口服液抗衰老的机制.方法 取自然衰老小鼠,给予活力苏口服液治疗,采用实时荧光定量PCR法观察小鼠肝、脑、肾组织p16基因的mRNA表达.结果 在肝、脑、肾组织中,活力苏口服液各剂量组均能降低p16基因mRNA表达,与衰老模型对照组比较均有显著性差异(P均<0.01).结论 活力苏能降低自然衰老小鼠肝、脑、肾组织p16基因的表达,发挥抗衰老作用.