IBV belongs to the corona virus γ group, can cause the chicken respiratory diseases, which can cause serious losses. Now there are many methods are widely used to diagnose IBV. This study used molecular biology technique to establish Real-Time PCR method for detecting IBV, the minimum detection sensitivity was 10 copies/μL, could effectively distinguish between other avian viruses; the difference of the same plate was 0.1%~0.7% and 0.7%~1.6% in different plates, prove this method has higher sensitivity, specificity and stability. At the same time we carried infec-tion experiment about the ck/HHLJ/HH13 isolated from wild strain, and tested its concentration. The results showed that there was higher concentration in trachea, lung and kidney in chickens about ck/HHLJ/HH13, and the concentration in trachea and kidney disappeared gradually until day 21 , and their pathological changes were seriously, at last which confirmed ck/HHLJ/HH13 isolated strain was a strong virulence nephridium IBV strain.%IBV（infectious bronchitis virus）属于冠状病毒γ群，可以引起鸡的呼吸道疾病，造成严重的损失，现在对IBV诊断方法的研究进行得十分广泛，建立了多种方法。本研究建立了IBV的Real-Time PCR检测方法，最低检测灵敏度为10 copies/μL，可以有效地区别其他禽源病毒，板内差异为0.1%～0.7%，板间差异为0.7%～1.6%，证明该方法具有较高的灵敏性、特异性和稳定性，并对分离到的野毒株ck/HHLJ/HH13进行了病毒感染试验的检测，在鸡体内的气管肺脏肾脏中都具有较高的浓度，其中气管和肾脏中病毒的滴度一直维持很高的水平，至感染后21 d才逐渐消失，病理组织学可见肾脏和气管病变最严重，证实了所分离到的野毒株ck/HHLJ/HH13是具有很强毒力的肾型IBV毒株。
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