马齿苋-肉桂联用提取工艺及其对DSS诱导溃疡性结肠炎的作用研究

摘要

目的:对马齿苋-肉桂联用最佳配比和多糖提取工艺及其多糖抗实验性溃疡性结肠炎 (UC) 作用进行研究.方法:采用L9 (34) 正交试验对马齿苋-肉桂联用最佳配比以及多糖的提取工艺进行优化;用3％葡聚糖硫酸钠 (DSS) 诱导小鼠UC模型, 灌胃300 mg·kg-1柳氮磺胺吡啶为阳性对照, 马齿苋-肉桂低中高实验组分别以100、200、400 mg·kg-1的多糖水溶液灌胃治疗, 观察小鼠给药前后的体征、结肠组织形态、血清白细胞介素-10 (IL-10) 及肿瘤坏死因子 (TNF-α) 的含量变化.结果:最佳提取工艺为马齿苋-肉桂12:5, 料液比1:30, 90℃提取5 h;给药治疗UC小鼠后, HE染色显示小鼠结肠黏膜上皮溃疡处有不同程度修复;给药组小鼠血清抑炎因子IL-10含量较模型组显著上升 (P<0.01), 促炎因子TNF-α含量较模型组显著降低 (P<0.01) .结论:本实验得出马齿苋配伍肉桂联用最佳配比和最佳提取工艺.给药治疗UC小鼠后, 从疾病活动指数 (DAI) 评分、HE染色、IL-10及TNF-α含量反映, 马齿苋-肉桂联用对UC有治疗作用, 为临床治疗UC提供了新思路.%Objective:To explore the optimal ratio of Portulaca oleracea combing with Cinnamomm cassia and evaluate their protecitive against DSS induced ulcerative colitis.Methods:Orthogonal experiment design L9 (34) was employed to optimize the extraction process.UC mice were induced by 3％ DSS and the DAI, H&E staining were enrolled to evaluated the protective effect of the extraction.The expression levels of IL-10 and TNF-α were also detected.Results:The best ratio of P.oleracea combing with C.cassia was 12:5.The extraction process was verified at 90 ℃ for 5 h.After administration of the extraction, the morphological structure of UC was improved and the inflammatory cells were almost disappeared, indicating the satisfied therapeutic effect.The expression level of IL-10 was greatly increased, while the pro-inflammatory cytokine TNF-α was decreased significantly.Conclusion:P.oleracea combing with C.cassia could improve the health conditions of UC mice via regulating the levels of inflammatory cytokines.And the extraction also can improve the morphological structure of UC.