丹皮酚在肝癌前病变模型大鼠体内的组织分布研究

摘要

目的:建立用超高效液相色谱(UPLC)测定肝癌前病变模型大鼠组织中丹皮酚的分析方法,研究丹皮酚在大鼠体内的组织分布特征.方法:采用UPLC测定大鼠组织中丹皮酚的浓度.结果:丹皮酚在对照组大鼠各组织中的分布顺序依次为肾＞胃＞小肠＞脑＞肝＞脾＞心＞肺,在模型组大鼠各组织中的分布顺序依次为肝＞肾＞脾＞肠＞胃＞心＞脑＞肺,且其在模型组各组织的AUCall均大于在对照组各组织的AUCal.结论:相对于对照组,丹皮酚在模型动物组织内含量增高.%Objective:To establish an analytical method for determination of Pae in tissues of hepatic precancerous lesion model rats by UPLC,and study the tissue distribution of Pae in rats.Methods:The concentration of Pae in rats tissues were determined by UPLC.Results:In control group,kidney had the highest tissue concentration of Pae,followed by stomach,small intestine,brain,liver,spleen,heart,lung;in model group,liver had the highest tissue concentration of Pae,followed by kidney,spleen,small intestine,stomach,heart,brain,lung.And its AUCall in model group was larger than that in control group.Conclusion:Compared with the control group,the content of Pae in model group increased.