何首乌及其主要成分二苯乙烯苷对非酒精性脂肪肝大鼠肠道短链脂肪酸产生量的影响

摘要

Objective:To study the effect of Radix Polygoni Multiflori and its active ingredient,TSG,on shortchain fatty acids (SCFA),which were gut microbiota fermentation products of carbohydrates,in rats with nonalcoholic fatty liver disease.Methods:SD rats were randomly distributed into seven groups,mice were fed with either standard chow diet or high-fat diet,plus daily administration of water or water extract of Radix Polygoni Multiflori or TSG.The contents of acetic acid,propionic acid and butyric acid in stool samples were determined by gas chromatography.TC,TG contents in liver and blood sample,and endotoxin contents in hepatic portal venous blood were tested.Results:The water extract of Radix Polygoni Muhiflori and TSG could reduce the contents of total SCFA in the intestinal tract of NAFLD mice fed by HFD,however,gender differences might existed.PMR and TSG could reduce acetic acid,propionic acid,butyric acid in HFD-fed male mice.At the same time,they relived high contents of TG,TC in liver tissue and LPS level in portal venous.In female rats,the propionic acid content was lower in PMR group compared with NAFLD rats.PMR also decreased the TC、TG level in hepatic tissues of female rats.However,low dose TSG raised acetic acid content,while reduced blood lipid and LPS level.In the meantime,regulation effect of PMRP on intestinal SCFA content of both female and male mice was not significant.Conclusion:Radix Polygoni Muhiflori and its active ingredient TSG affected SCFA level produced by gut microbial fermentation.This regulatory activity may contribute to their treatment activity to NAFLD.%目的:探讨何首乌及其主要成分二苯乙烯苷(TSG)对非酒精性脂肪肝模型大鼠肠道内微生物发酵碳水化合物产生短链脂肪酸量的影响.方法:SD大鼠随机分为正常组、高脂饮食组和高脂饮食给药组(分别给予何首乌水提物和TSG).采用气相色谱法检测各组大鼠粪便中短链脂肪酸(以乙酸、丙酸、丁酸为代表)含量.COD-PAP法测定血液和肝脏总胆固醇(TC)、甘油三酯(TG)含量,鲎试验法检测肝门静脉血内毒素.结果:何首乌总提物及TSG各剂量都能不同程度的降低高脂饮食大鼠肠道内的总短链脂肪酸(SCFA)含量,但其调节作用可能存在性别差异.生何首乌及TSG能够显著降低高脂饮食雄性大鼠肠道内乙酸、丙酸、丁酸含量,同时降低实验动物肝脏脂质含量和内毒素水平.生何首乌下调高脂饮食雌性大鼠肠道内丙酸含量,降低肝脏脂质水平;而TSG低剂量能够升高乙酸含量,同时降低血脂和内毒素含量.然而制何首乌对雌、雄鼠肠道内SCFA含量的调节作用均不显著.结论:何首乌及其活性成分TSG能够调节肠道微生物发酵产生的短链脂肪酸含量,这种调节活性可能与其对非酒精性脂肪肝的治疗作用有关.