潺槁树根皮化学成分及其抗糖尿病靶点筛选研究

摘要

目的:对潺槁树根皮化学成分进行研究,并探索化合物降糖作用靶点.方法:采用硅胶、SephadexLH-20等柱色谱以及半制备HPLC进行分离,选择14个与糖代谢相关靶点蛋白,采用分子对接技术探索化合物降糖作用的靶点.结果:从潺槁树根皮中得到6个化合物,经波谱学方法鉴定为N-反式-阿魏酰酪胺(1)、N-顺式-阿魏酰酪胺(2)、N-反式-芥子酰酪胺(3)、锡兰肉桂醇(4)、波尔定碱(5)、新木姜子碱(6).分子对接结果表明,化合物5、6与蛋白酪氨酸磷酸酶(PTP1B)结合度最高.结论:化合物1～4为首次从该属植物分离得到,化合物5、6可能通过调控PTP1B发挥功效.%Objective:To study the chemical constituents from the bark of Litsea glutinosa and to investigate their antidiabetic targets in silico.Methods:Silica gel and Sephedex LH-20 column chromatographies as well as semi-preparative HPLC were applied to isolate and purify the compounds.Molecular docking was used to evaluate the binding activities of the ligands and the 14 selected proteins.Results:Six compounds were obtained and identified as N-trans-feruloyltyramine (1),Ncis-feruloyltyramine (2),N-trans-sinapoyltyramine (3),cinnzeylanol (4),boldine (5),laurolitsine (6).Molecular docking results showed that compounds 5 and 6 banded well with PTP1 B.Conclusion:Compounds 1-4 were isolated from genus L.glutinosa for the first time.Compounds 5 and 6 may exert their biological activities through regulating PTP1B.