高效液相色谱法测定酰嘧磺隆、乙氧磺隆、烟嘧磺隆原药的含量

摘要

A method for the determination of oxine-copper 33.5% SC was developed by GC with Elite-17 capillary column, FID detector and dimethyl phthalate as internal standard. The results showed that the linear correlation coefficient was 0.9998, the variation coefficient was 0.49%, and the average recovery was 99.3%. The method is simple and suitable for routine analysis and quality control of oxine-copper 33.5% SC.%对以20 mmol/L甲酸+10 mmol/L甲酸铵作为调节剂,采用乙腈/水为流动相,C8柱分离,PDA检测器测定酰嘧磺隆、乙氧磺隆、烟嘧磺隆原药的高效液相色谱分析方法进行了研究.酰嘧磺隆以苯甲酸甲酯为内标,乙腈比例40％,230 nm检测;乙氧磺隆以邻苯二甲酸二甲酯为内标,乙腈比例55％,222 nm检测;烟嘧磺隆以邻苯二甲酸二甲酯为内标,乙腈比例30％,232 nm检测.在25～500 mg/L范围内,线性方程分别为Y=1.1042 X+0.0152,Y=0.7606 X+0.0064,Y=1.1224 X+0.005 6.r值分别为0.9999,0.999 8,0.9992.添加回收率分别为99.37％～99.43％, 99.09％～99.38％,99.79％～99.84％.RSD分别为0.85％,0.46％,0.27％.建立的3种嘧啶磺酰脲类除草剂原药的内标定量分析方法可将原药及杂质/降解产物同时分离,定量结果准确可靠.