大肠杆菌基因组水平蛋白质-RNA相互作用初步研究

摘要

目的：初步研究大肠杆菌中基因组水平的蛋白质-RNA相互作用（ protein-RNA interactions，PRI）。方法通过RNA酶消化细菌裂解液，提取与蛋白质相互作用的RNA片段，构建cDNA文库，进行高通量测序，并通过生物信息学分析获得与蛋白质结合的转录本。结果获得了与蛋白质结合的3193条转录本，涉及2234个mRNA、47个sRNA（small regulatory RNAs）、39个tRNA、11个rRNA以及862个基因间区（intergenic region， IGR）。结论初步获得大肠杆菌中与蛋白质相互作用的转录本信息，为进一步开展PRI研究提供了支持。%Objective To conduct a pilot study on genome-wide in vivo protein-RNA interactions in E.coli.Methods Bacterial lysate was treated with RNase before the RNA fragments protected by proteins were extracted from treated lysate and used to construct cDNA library that was applied to high-throughput sequencing .Finally, the transcripts bound by proteins were obtained by bioinformatics analysis .Results A total of 3193 transcripts were obtained , including 2234 mRNAs, 47 sRNAs, 39 tRNAs, 11 rRNAs, and 862 intergenic regions .Conclusion Some information of transcripts interacting with proteins in E.coli is acquired , which will facilitate further studies of protein-RNA interactions .