不同方法提取寡聚RNA-DNA杂合体效果比较

摘要

Objective To compare the effects of three different methods for extracting short RNA-DNA hybrids, including the TRI reagent method , the phenol saturated with water method and the phenol saturated with Tris buffer method in order to facilitate studies on the biological function of RNA-DNA hybrids .Methods Short RNA fragments modifiedwith FAM at the 5′end and those modified with Cy 5 at the 5′end were synthesized .RNA and DNA fragments were annealed to form RNA-DNA hybrids.They were extracted with the above-mentioned 3 methods respectively .The extracted products were analyzed with electrophoresis .Results and Conclusion Short RNA-DNA hybrids can be extracted by the phenol saturated with water method and by the phenol saturated with Tris buffer method .The results can help study the function of short RNA-DNA hybrids .%目的 比较TRI试剂法、水饱和酚法和Tris饱和酚法提取小RNA-DNA杂合体片段的效果,为开展RNA-DNA杂合体的生物学功能研究提供方法.方法 合成5′端荧光分子修饰的短RNA和DNA片段,形成小RNA-DNA杂合体.分别采用上述3法提取寡聚RNA-DNA杂合体,电泳分析提取结果.结果与结论 水饱和酚和Tris饱和酚法均能提取到小RNA-DNA杂合体. 该研究为小RNA-DNA杂合体功能研究和检测分析提供了基础.