流式细胞术检测对阵发性睡眠性血红蛋白尿症的诊断价值

摘要

Objective To investigate the value of flow cytometry in the diagnosis of paroxysmal nocturnal hemoglobin (PNH).Method 61 patients with anemia were selected in October,2015 March.2013 in our hospital.According to the condition,the patients were divided into PNH group (24 cases with nocturnal hemoglobin),group AA (24 cases with aplastic anemia),group MDS(4 cases with bone marrow hyperplasia syndrome) and other anemia group (18 cases with other anemia).Flow cytometry was used to detect patients with peripheral blood erythrocytes,granulocytes membrane CD55 and expression of CD59.Results The expression of CD55 and CD59 in the red blood cells,the membrane of the PNH group was significantly higher than the other three groups (P＜0.05).There was no significant difference between the AA group and the MDS group (P＜0.05).The expression of CD55 and CD59 in erythrocyte,granulocyte and membrane were all less than 10％ in other anemia patients,and the difference was statistically significant (P＜0.05).Conclusion Detection of PNH by flow cytometry is highly specific,rapid and accurate,and can effectively help the diagnosis of PNH,which can be used in clinical application.%目的 探讨流式细胞术检测阵发性睡眠性血红蛋白尿症(PNH)的诊断价值.方法 选取于2013年3月～2015年10月在我院就诊的贫血患者61例,按照病情分为阵发性睡眠性血红蛋白尿症组(PNH组)24例、再生障碍性贫血组(AA组)15例、骨髓增生异常综合征组(MDS组)4例和其他贫血组18例,用流式细胞术检测患者外周血红细胞、粒细胞膜CD55和CD59的表达情况.结果 PNH组患者红细胞、粒细胞膜CD55和CD59表达量显著大于其他3组,差异均有统计学意义(P＜0.05),AA组和MDS组间比较差异无统计学意义(P＜0.05);而其他贫血组患者红细胞、粒细胞膜CD55和CD59表达均＜10％,且明显低于其他3组,差异均有统计学意义(P＜0.05).结论 流式细胞术检测PNH特异性强,快速,准确,能有效帮助诊断PNH,可在临床推广应用.