Objective To explore a new method for establishing the rabbit model of alveolar high altitude pulmonary edema ( AHAPE ) in a high altitude environment. Methods 36 rabbits were carried from Chongqing to Lhasa ( 3700 m above sea level ) in winter. They were randomly divided into four groups: normal control group ( group N ), dexamethasone group ( group D ), propranolol group ( group P ), sleep interference group ( group G ). All animals were transfused with 200 ml of 37 ℃ normal saline at a speed of 13.3 ml/min. 20 min later, 200 ml of the same saline were transfused again, and the total amount of transfusion was 400 ml. In addition, group D was injected with dexamethasone at 2 mg/kg daily; group P was intragastrically administered with propranolol at 2. 5 mg/kg daily;group G was given alarum three times and illumination at night;group N was routinely fed without sleep interference or drug administration. Blood pressure, central venous pressure, respiratory and heart rate were monitored during the experiment. Results AHAPE occurred in 3 rabbits in group G and 2 rabbits in group P,while no HAPE case was found in group N and D. Pulmonary water content ( PWC ) in group G was much higher than that in group N( P ＜0. 01 ). Compared with group N,group P and D showed a slight decrease in PWC but the differences were not statistically different. Conclusion An AHAPE model is successfully duplicated at 3700 m high altitude by normal saline transfusion combined with sleep interference or with propranolol which can influence pulmonary water reabsorption.%目的 探讨在高原环境下建立高原肺水肿(HAPE)动物模型的方法.方法 将大白兔随机分为对照组(N)、地塞米松组(D)、普萘洛尔组(P)、睡眠干扰组(G),在海拔3700 m高原冬季环境下,给动物以13.3ml/min速度输37℃生理盐水200ml,20min后再输200ml,共输400 ml.试验中观察动物血压、中心静脉压、呼吸、心率等情况.结果 G组3只发生HAPE,P组2只,而N组和D组没有发生;G组的肺含水量比N组明显增高(P<0.01),P、D组肺含水量比N组减少,但差异无统计学意义.结论 在高原现场,通过输液诱导方式,在药物影响肺重吸收功能和睡眠干扰情况下,复制出了肺泡型HAPE模型.
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