补锌对缺锌大鼠烧伤早期肠粘膜通透性的影响

摘要

Objective:To investigate the effect of zinc supplements on gut mucosal permeability in zinc dificient rats after burns.Methods:Weanling rats were pair-fed zinc deficient diets (zinc content:1.6 μg*g－1)for one week to induce zinc deficient status,t hen partial thickness of 20％ TBSA was made.Then the rats were divided into thre e groups fed on chows different in zinc contents.Zinc contents were 1.6 μg*g －1，24.7 μg*g－1,286.9 μg*g－1respectively.A group of rats fed normal zinc content(24.7 μg*g－1)diets before and after scalds was d esigned as control.Lactose(L) and mannitol(M) were force-fed one day before and one day after scalds were made.The amounts of L and M as well as the ratio of L ／M in urine were detected to reflect the permeability of gut mucosa. Results:The ratio of L／M was higher in zinc deficient rats (0 .2 370±0.0 105) than that in control group (0.1 202±0.0 146)before scald.It wa s resulted from higher concentration of L in urine of the former.The amounts of L and M in urine were decreased to different extent after different amounts of z inc were supplemented.It was more evident in the group fed high zinc content cho ws(0.1 704±0.0 565).Conclusion:The nutritional stat us of zinc has some effects on the permeability of gut mucosa.To supplement zinc after major burns could decrease the increased permeability of intestinal mucosa.%目的：探讨缺锌与补锌对大鼠烧伤早期肠粘膜通透性的影响。方法：1个月龄大鼠进食低锌饲料(含锌量：1.6 μg*g－1)1周造成缺锌模型，20％深Ⅱ度烫伤后改饲3种不同含锌饲料(含锌量分别 为：1.6、24.7和286.9 μg*g－1)。同时设1组大鼠烫伤前后均进食正常含锌饲料( 含锌量：24.7 μg*g－1)，做对照组。在伤前1天与伤后第1天以甘露醇、乳果糖混 合溶液灌胃，气相色谱法测定二者在尿中的含量和比例反映肠粘膜通透性的大小。结果：伤前进食低锌饲料的大鼠尿中乳果糖／甘露醇之比(0.2 3 70±0.0 105)显著高于对照组(0.1 202±0.0 146)，主要由尿中乳果糖含量较高引起；伤 后改饲含锌饲料使尿中甘露醇、乳果糖的含量、比例均降低，尤以改饲高锌饲料组效果显著 (0.1 704±0.0 565) 。结论：锌营养状态影响肠粘膜通透性，补锌可使烧伤后 升高的肠通透性下降。