活性羰基化合物对人血管内皮细胞粘附分子表达的影响

摘要

为探讨活性羰基化合物蓄积在慢性肾功能衰竭和糖尿病血管并发症发病机制中的作用，应用流式细胞仪检测3-脱氧葡糖醛酮和甲基乙二醛对人血管内皮细胞表达粘附分子的影响。结果显示，3-脱氧葡糖醛酮和甲基乙二醛均可上调人血管内皮细胞对ICAM-1和VCAM-1的表达，并呈时间和剂量依赖关系；羰基化合物清除剂（氨基胍）对上述羰基化合物产生的上调效应具有抑制作用。结果提示，活性羰基化合物可能与慢性肾衰和糖尿病血管病变的形成有关。%To elucidate the role of reactive carbonyl compounds in the pathogenesis of vascular complication in patients with chronic renal failure or diabetes. Vascular endothelial cells (VECs) were isolated from human umbilical vein and cultured with 3-deoxyglucosone (3-DG) or methylglyoxal(MGO) in vitro. Expression of ICAM-1 and VCAM-1 on the surface of VECs was detected by flow cytometer. The results showed that up-regulation of expression of ICAM-1 and VCAM-1 was observed when either 3-DG or MGO was added into the cultures, which was inhibited by a carbonyl compounds scavanger, aminoguanidine. These data suggested that accumulation of reactive carbonyl compounds in patients with chronic renal failure or diabetes might be involved in the mechanism of arteriosclerosis.