HBV转基因小鼠血液生理生化指标检测分析

摘要

Objective To study the effects of gene integration of HBV on the biochemical and hematological indices in blood of transgenic mice. Methods The venous blood was collected from orbital venous plexus of 28 normal mice bom in the same brood (HBsAg negative) and 50 HBV transgenic mice (HBsAg positive), 6-8 weeks in age The blood routine examination was performed, including white blood cells (WHO, red blood cells (RBC), hemoglobin (Hb), platelets (PLT), lymphocyte percentage (L%), intermediate cell percentage (M%), the percentage of leaf cells (G%) and blood biochemical parameters including glucose (GLU), urea nitrogen (BUN), creatinine (CREA), total protein (ALB), albumin (ALB), total bilirubin (TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (CHOL) and triglyceride (TG). The differential indexes were analyzed in HBsAg positive and negative mice, also the mice of different sex. Results Significant differences were found between the transgenic and normal mice in blood GLU, BUN, CREA, RBCs, Hb and PLT (P<0.05), while no significant differences were found in other indices. No difference was found in the above 6 indices between the different sex of normal mice, while there was significant difference in blood GLU between males and females in transgenic mice, and it was higher in males than in females ( P<0.05). The blood GLU, CREA and BUN were higher in transgenic male mice than in normal male mice, and the contents of RBCs, Hb and PLT were higher in transgenic female mice than in normal female mice (P<0.05). Conclusion HBV DNA may throw some influences on blood biochemical and hematological indexes of HBV transgenic mice, and it may provide some valuable references to the study of HBV pathogenesis in HBV animal models.%目的 研究HBV基因整合对转基因小鼠代谢和血液指标的影响.方法 以28只同一窝乙肝表面抗原(HBsAg)阴性小鼠和50只HBsAg阳性的HBV转基因小鼠为研究对象,鼠龄6～8周,眼眶静脉丛采血,检测血液常规指标白细胞(WBC)、红细胞(RBC)、血红蛋白(Hb)、血小板(PLT)及淋巴细胞百分比(L%)、中间细胞百分比(M%)、分叶细胞百分比(G%),血液生化指标葡萄糖(GLU)、尿素氮(BUN)、肌酐(CREA)、总蛋白(ALB)、白蛋白(ALB)、总胆红素(TBIL)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆固醇(CHOL)、甘油三酯(TG)等,并对有差异的指标进行品种(HBsAg阳性鼠和阴性鼠)内及性别间比较分析.结果 转基因小鼠血糖、尿素氮、肌酐、红细胞、血红蛋白、血小板指标与正常小鼠之间有显著差异(P＜0.05),而其余指标无明显差异(P＞0.05).上述6项指标在不同性别正常小鼠之间没有差异,而在不同性别转基因小鼠之间仅血糖存在差异,雄鼠血糖高于雌鼠(P＜0.05).转基因雄鼠血糖、肌酐、尿素氮明显高于正常雄鼠,而转基因雌鼠红细胞、血红蛋白、血小板指标明显高于正常雌鼠(P＜0.05).结论HBVDNA对小鼠的生理生化指标有一定影响.