人正常宫颈组织 CD3、CD4、CD8的免疫组织化学定量分析

摘要

Objective:To observe distribution and number of T cells in human normal cervical tissue and provide experimental data for further studies like expressions of T cells in cervical lesions and cervical cancer tissues. Methods:The number, distribution and expression intensity of the surface differentiation antigen CD3, CD4, CD8 positive T cells in the normal cervical tissue were detec-ted by immunohistochemical SP technique and computerized imaging analysis system. Results:The CD3 positive cells mainly distribu-ted in the cervical mucosal epithelium and connective tissue above the adjacent lamina propria. The CD8 positive cells widely distribu-ted in the human cervical mucosal lamina propria connective tissue. The CD4 positive cells mainly distributed in the lamina propria connective tissue under the human cervical epithelium and the connective tissue around the cervical mucosa adenoid fossae. The num-ber of CD4 positive cells was higher than that of CD8 positive cells. Conclusions: In the human normal cervical tissue, The CD3, CD4, CD8 positive cells maintain a certain proportions.%目的：观察人正常宫颈组织中T 细胞的分布、数量，为深入探讨宫颈病变组织、宫颈癌组织中相关T细胞的表达等提供实验资料。方法：采用免疫组织化学SP法和图像分析技术，检测人正常宫颈组织中表面分化抗原CD3、CD4、CD8阳性T细胞的分布、数量和表达强度。结果：CD3阳性细胞主要分布于人子宫颈黏膜上皮及近固有层结缔组织内。 CD8阳性细胞广泛分布于人子宫颈黏膜固有层结缔组织内。CD4阳性细胞主要分布于人子宫颈黏膜上皮下固有层结缔组织内和宫颈黏膜腺样隐窝周围的结缔组织内。 CD4阳性细胞数略高于CD8阳性细胞。结论：人正常宫颈组织中含有一定的比例的CD3、CD4、CD8阳性T细胞。