ETV1在胃肠道间质瘤中的特异性表达

摘要

Objective:To investigate the expression of ETV1 in gastrointestinal stromal tumors and its clinical significance.Method:Surgical gastrointestinal stromal tumor tissues and adjacent normal mucosa were obtained from 135 patients with gastrointestinal stromal tumor.Immunohistochemistry was conducted to detect the distribution of ETV1 protein,while the quantitative real-time reverse transcriptase polymerase chain reaction and Western blot method were used to detect the expressions of ETV1 mRNA and protein.Result:Immunohistochemical analysis showed the positive rate of ETV1 expression was 68.1% in gastrointestinal stromal tumor tissues,it was significantly higher than 5.2% in the adjacent normal samples(P<0.05).Fluorescence quantitative polymerase chain reaction (PCR) showed that relative expression of Ct value was (3.30±2.37)in gastrointestinal stromal tumor tissues,it was significantly lower than (6.19±2.86) in adjacent normal samples,expression of ETV1 in tumor tissue was 7.41 times of normal tissue,the difference was statistically significant(P<0.001).Western blot analysis revealed that the expression of ETV1 protein was (1.24±0.67) in gastrointestinal stromal tumor tissues,it was significantly higher than (0.88±0.28) in normal colorectal tissues (P=0.0369).Pearson correlation analysis showed that the mRNA level was in positive correlation with the protein level(r=0.526,P=0.017).Conclusion:The specific expression of the ETV1 is in a correlation with the pathogenesis of gastrointestinal stromal tumor.ETV1 is expected to be a new tumor marker and the new target for clinical therapy.%目的：探讨ETV1在胃肠道间质瘤组织中的表达及临床意义。方法：应用免疫组化、实时荧光定量PCR、Western-blot检测ETV1在135例不同胃肠道间质瘤患者的肿瘤组织及瘤旁组织中的表达。结果：免疫组化结果显示，ETV1在胃肠道间质瘤中阳性表达率为68.1%，显著高于瘤旁正常组织的5.2%（P<0.05）。荧光定量PCR方法显示，间质瘤组织中ETV1的相对表达Ct值（3.30±2.37）低于瘤旁正常组织的（6.19±2.86），肿瘤组织中ETV1的表达是正常组织的7.41倍，差异有统计学意义（P<0.001）。Western-blot法检测显示， ETV1蛋白在胃肠道间质瘤中的表达水平（1.24±0.67）也较瘤旁正常组织高（0.88±0.28）（P=0.0369）；Pearson相关分析显示，ETV1mRNA和蛋白水平呈正相关（r=0.526，P=0.017）。结论：ETV1在胃肠道间质瘤中特异性高表达，可能与胃肠道间质瘤的发生发展有关，有望成为新的肿瘤标志物，为临床治疗提供新靶点。