血细胞分析仪分析结果异常时与血涂片的临床对比研究

摘要

Objective:The specimen smears were stained by the blood cell analyzer,and the number, form and classification of blood cells were compared to those results of blood cell analyzer to verify if the results of the blood cell analyzer are reliable and accurate.Method:Two thousand specimens of patients showing abnormal blood cell analysis results via the blood cell analyzer admitted from June 2013 to June 2015 were subjected to research and analysis,and such results were subject to analysis and comparison to results determined via blood smear microscope.Result:The number of platelets of patients were higher than that of normal specimen smears, being the same to results of the blood cell analyzer,but the number of partial platelets didn’t coincided with normally lower part,pseudo thrombocytopenia resulted from the aggregation of platelets must be subjected to blood smear detection.The results of RBC volume tested via blood smear microscope were the same to those via blood cell analyzer.Abnormal WBC specimens classified or unclassified were required to subject to blood smear microscopy. Conclusion:Testing results by using of blood cell analyzer are abnormal and not completely reliable,only the comparison of smear staining microscope test results can the disadvantage of the blood cell analyzer failing to correctly identify abnormal cell compositions,accurate and scientific results are obtained finally,decreasing the misdiagnosis probability of patients.%目的：将血细胞分析仪所检测出现异常的标本涂片染色，在显微镜下对其血细胞数量、形态、分类与血细胞分析仪检查结果实施对比，以此检验血细胞分析仪分析的结果是否可靠与准确。方法：将本院2013年6月-2015年6月接收的通过血细胞分析仪出现血细胞分析结果异常的患者标本2000例进行研究分析，将其与血涂片镜检测的结果实施分析对比。结果：血小板数量比正常标本涂片较高，与血细胞分析仪分析的结果相同，但部分血小板数量比正常低的部分不符合，因其部分血小板的聚集导致出现假性血小板减少，必须给予血涂片镜检测；血涂片镜检测和血细胞仪分析的红细胞体积结果相同；对分类或不分类异常的白细胞标本须给予血涂片镜检。结论：应用血细胞分析仪所检测的结果出现异常、不完全的可靠，只有将涂片染色镜检测结果对比，才可将血细胞分析仪不可正确识别异常细胞成分的缺点进行修正，最后得到准确而又科学的结果，降低患者的漏误诊率。