水相中L-组氨酸单分散分子印迹聚合物微球的合成、表征及其识别性能研究

摘要

The monodisperse molecular imprinting microspheres using L-histidine, methyl-acrylic acid(MAA)/ or acrylic aimde(AM), ethylene glycol dimethacrylate(EDMA) as template molecule, functional monomer and cross liker respectively were synthesized by soap-free emulsion polymerization technique under the optimal condidtion in aqueous phase The morphology, particle size and its distribution of the microspheres of L-histidine molecular imprinting polymers and the interaction between the function monomer and the template molecule as well as the co-polymerization between the function monomer and the cross liker were characterized by meams of SEM, laser particle analyses and ultraviolet as well as FTIR respectively. Their adsorption capaccity was evaluated by measured their separation factor(α) and relative separation factor(β)through static adsorption technique The experimental results show that the L-histidine molecular imprinting polymer microspheres synthesized by this technique exhibit better adsorption selectivity and better adsorption capacity to the substrate.%较佳工艺条件下,在水性体系中选用无皂乳液聚合法制得的单分散微米级聚苯乙烯微球为种球,分别以组氨酸、甲基丙烯酸(MAA)或丙烯酸胺(AM)、乙二醇二甲基丙烯酸酯(EDMA)为模板分子、功能单体和交联剂,合成了L组氨酸分子印迹聚合物微球.研究了形貌、粒径及其分布以及模板分子与功能单体之间的相互作用,分别以SEM、激光粒度分析仪紫外分光光度法和红外光谱表征功能单体与交联剂之间的共聚情况,通过静态吸附法分别测定其分离因子(α)和相对分离因子(β)来评价其吸附性能.结果表明,该法合成的分子印迹聚合物微球对底物具有较高的吸附选择性和较大的吸附容量.