首页> 中文期刊>海洋通报 >中国近海角木叶鲽(Pleuronichthys cornutus)种群遗传多样性研究

中国近海角木叶鲽(Pleuronichthys cornutus)种群遗传多样性研究

     

摘要

测定了中国沿海角木叶鲽4个群体57个体的线粒体控制区序列,在长度为549 bp的线粒体控制区序列中,检测到3个插入/缺失位点,24个变异位点(包括11个简约信息位点),共出现了37个单倍型,其中9个为共享单倍型.角木叶鲽整体的单倍型多样度(Hd)和核苷酸多态度(Pi)结果分别为0.979±0.008和0.00655±0.0037,呈现出高单倍型多样度和低核苷酸多态度分布模式.中性检验结果显示,角木叶鲽种群的Fu's Fs为显著负值,核苷酸不配对分析呈现单峰分布,表明角木叶鲽在历史上经历过种群扩张事件.群体内个体间的平均遗传距离为0.0065,群体间遗传分化指数都为负值(-0.0067~-0.0460)但不显著(P>0.1),AMOVA分析显示遗传变异主要集中在群体内个体间(102.15%),均表明我国近海角木叶鲽种群无明显的分化,可以作为一个渔业管理单位加以保护和利用.%The length of 549 bp mtDNA control region sequences of 57 individuals from 4 Pleuronichthys cornutus populations in the coastal waters of China was determined, 3 insert/deletion sites, 24 variable sites (including 11 parsinomy-informative sites) and 37 haplotypes were detected, of which 9 haplotypes were shared among populations. The global haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.979 0.008 and 0.0061 0.0037 respectively, indicating a high haplotype diversity (Hd) and a low nucleotide diversity {Pi). The significant negative values of neutral test of Fu's Fs and the unimodal mismatch distribution revealed a historical population expansion. As the average genetic distances within 4 populations were 0,0065, and genetic differentiation index (Fst) were negative (-0.0067~-0.0460) but not significant (P > 0.1), and the majority eenetir variation (102.15%) occurred within populations (102.15%) in AMOVA analysis, suggesting that Pleuronichthys cornutus populations in the coastal waters of China were not significantly differentiated and might be protected as a single management unit.

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