To investigate the clinical significance of TDGF-1 expression and the correlation between TDGF-1 expression and epithelial-mesenchymal transition (EMT) in gastric cancer. Immunohistochemical SP and RT-PCR methods were used to examine the expression of TDGF-1, E-cadherin, Vimentin proteins and mRNA in gastric cancer and normal gastric tissues (IHC-70 cases; RT-PCR-40 cases). Then the association between TDGF-1 expression and clinical pathological characteristics as well as the correlation between expression of TDGF-1, E-cadherin, Vimentin were analyzed. The positive expression rates of TDGF-1, E-cadherin, Vimentin protein in gastric cancer were 70%, 34.3% and 52.9% respectively, but the positive expression rates of them in normal gastric tissues were 38.5%, 94.3% and 15.7%(P<0.05) respectively. The positive expression rates of TDGF-X, E-cadherin, Vimentin mRNA in gastric cancer were 67.5%, 37.5% and 52.5% respectively, but the positive expression rates of them in normal gastric tissues were 35%, 87.5% and 22.5% (P<0.05) respectively. The expression of TDGF-1 protein and mRNA were significantly associated with depth of invasion, lymph node metastasis and TNM stages (P<0.05). The expression of TDGF-1 was closely corre-lated with that of E-cadherin (r=-0.447, P<0.05); Moreover, the expression of TDGF-1 was closely correlated with that of Vimentin (r=0.318, P<0.05). On the other hand, the expression of E-cadherin was closely correlated with that of Vimentin (r=-0.283, P<0.05). TDGF-1 may promote EMT through the regulation of the expression of E-cadherin and Vimentin, thereby clearly showing that it plays an important role in invasion and metastasis of gastric cancer.%探讨胚胎瘤衍生生长因子(teratocarcinoma-derived growth factor-1,TDGF-1)在胃癌中表达的临床意义及其与上皮间质转化(epithelial-mesenchymal transition,EMT)的关系.分别采用免疫组化SP法和逆转录-聚合酶连反应(RT-PCR)方法,检测胃癌组织和正常胃组织中TDGF-1、E-cadherin、Vimentin的表达情况(IHC70例；RTPCR40例),分析TDGF-1表达与临床病理特征的关系及TDGF-1、E-cadherin、Vimentin三者表达的相关性.TDGF-1、E-cadherin、Vimentin蛋白在胃癌组织中的阳性表达率分别为70％、34.3％、52.9％,在正常胃组织中的阳性表达率分别为38.5％、94.3％、15.7％ (P＜0.05); TDGF-1、E-cadherin、Vimentin mRNA在胃癌组织中的阳性表达率分别为67.5％、37.5％、52.5％,在正常胃组织中的阳性表达率分别为35％、87.5％、22.5％ (P＜0.05); TDGF-1蛋白和mRNA的表达均与胃癌的浸润深度、淋巴结转移及TNM分期显著相关(P＜0.05); TDGF-1高表达与Ecadherin表达减低显著相关(r=-0.447、P＜0.05),TDGF-1高表达与Vimentin表达升高显著相关(r=0.318、p＜0.05),E-cadherin表达减低与Vimentin表达升高显著相关(r=-0.283、P＜0.05).TDGF-1可能通过调控E-cadherin、Vimentin表达促进EMT,从而在胃癌的侵袭转移中发挥重要作用.
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