将携带p53基因的复制缺陷型腺病毒载体Adp53导入到p53完全缺失的人肝癌细胞株中,培养人肿瘤细胞,并分别加入5μmol/L顺铂(Pt)作用24h,以RT-PCR技术检测FrpHE mRNA的表达作用,以流式细胞术检测Adp53的转基因情况和β-catenin的改变为功能指标评价Wnt通路的变化.FrpHE mRNA表达水平在转染Adp53和作用Pt 24 h后即有明显升高,在人大肠癌细胞和人神经胶质瘤细胞中,未见FrpHE mRNA表达.β-catenin表达水平逐渐下降.提示外源性物质p53和顺铂能明显诱导FrpHE的表达,进而产生抑制Wnt通路的作用.%Human hepatocarcinoma cells were transfected with a replication-defective advenovirus encoding (Adp53), human tumor cells were transfected with antitumor-drug cisplatin after 24 h, Adp53 and /3-cafenm expression was measured by fluorescence-activated cell sorting (FACS) and then Wnt signaling pathway inhibitor FrpHE expression was detected by RT-PCR. FrpHE mRNA was initially potentiated by Adp53 and cisplatin after 24 h, no inhibitor FrpHE was expressed in Lovo and U251, /3-catenin expression levels was descent. Extogenous materials p53 and cisplatin induced expression of FrpHE and suppress Wnt signaling pathway.
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