基于气相色谱法检测食品中对羟基苯甲酸酯类残留量

摘要

In this paper,a method to detect the parabens(ethyl and propyl) from food by Gas Chromatography was established. After extracted by a mixed extractant(n-hexane + diethylether,volume ratio 1:9) under acidic condition,the sample was washed with sodium bicarbonate solution,separated with a HP-5 melted quartz capillary column and detected by a flame ionization detector.The retention time was observed for qualitation and external standard was used for quantitation.The correlation coefficient of ethyl p-hydroxybenzoate is 0.9992 among the concentration range of 50~800μg/ml,the recoveries are between 89.95%to 98.35%and the relative standard deviation is 3.29%.This method shows a high degree of accuracy,good stability,and high sensitivity.%本文建立了一种采用气相色谱法检测食品中对羟基苯甲酸酯类（乙酯和丙酯）含量的方法。样品在酸性条件下用混合萃取剂（正乙烷＋乙醚,体积比1：9）萃取后,经碳酸氢钠溶液洗涤净化,浓缩定容,用HP-5熔融石英毛细管柱分离并用氢火焰检测器检测。采用保留时间定性和外标法定量的分析方法进行测定。对羟基苯甲酸乙酯在50～800μg/ml的浓度范围内,线性相关系数R为0.9992,加标回收率在89.95%～98.35%之间,相对标准偏差为3.29%;对羟基苯甲酸丙酯在50～800μg/ml的浓度范围内,线性相关系数R为0.9997,加标回收率在94.30%～102.11%之间,相对标准偏差为2.83%。该方法测定结果准确可靠,且稳定性好,灵敏度高。