流体中血红细胞光散射信息的建模与仿真

摘要

In order to obtain accurate light scattering information of red blood cell in the fluid flow , the scattering model of red blood cell in the fluid flow was constructed based on the anomalous diffraction approximation and the oblate ellipsoid.The effect of the variety of body, volume, relative refractive index and orientation angle on the scattering amplitude was studied.The simulation data indicate that the volume and relative refractive index of different red blood cell has the prominent effect on the scattering information at small orientation angles .The model was proved by experiments . This result is helpful for measuring red blood cell fast and accurately and the precise measurement of the cell measurement instrument such as flow cytometer.%　　为了获取流体中血红细胞精确的光散射信息，以反常衍射近似理论为基础，采用扁椭球构建了流体中血红细胞的数学散射模型。应用数值仿真模拟技术，分别讨论了血红细胞形体变化、体积变化、取向角和相对折射率变化对光散射幅值分布的影响，建立了相应的图谱分布，并通过实验对此模型进行了验证。结果表明，在前向小角度内，不同血红细胞大小、不同相对折射率对光散射信息有着较大影响。这一结果对于病理血红细胞的快速准确检测和流式细胞仪等细胞测量仪的精确测量是有帮助的。