Objective To understand the structure and variation regulation of human papillomavirus (HPV ) type 58 L1 gene in Putian city ,Fujian province ,in order to provide theoretical basis for clinical treatment and preven-tion .Methods Nucleic acids rapid diversion genotyping chip(HybrMax) was used in HPV genotype .Four strains of type HPV58 positive samples was randomly selected ,then cloned and sequenced ;further results sequenced and 32 strains of HPV type 58 virus strains L1 gene nucleotide sequence in Gen Bank were used to establish homology ma-trix ,analyzed and build phylogenetic tree construction by DNAstar ,clustalx 2 and Mega5 .0 software .Results The gene of these four strains of HPV virus type 58 strains shared highly homology 96% - 98% in nucleotide with each other ,92% - 98% in protein level too ;with 1986 French line ,nucleotide homology is 83% - 86% ,the homology of a-mino acid is 84% - 89% ;with 1987 the British lines ,nucleotide homology is 53% - 54% ,the amino acid sequence is 39% - 42% ;with separation and the 2010 Hongkong ,2004 Jiangsu isolates nucleotide strains ,homology is 96% -100% ,the homology of amino acid is 92% - 99% ,the highest homology .From the phylogenetic tree diagram ,the gene background of these 4 strains of HPV58 virus in the region and that of the EV999963 ,DQ057326 ,HM639611 strains virus from Shanghai ,Hongkong were most closely .Conclusion The region type HPV58L1gene has certain regional characteristics .%目的：了解莆田地区人乳头状瘤病毒（HPV）58型 L1基因结构特点和变异规律，为临床治疗和预防提供理论依据。方法采用核酸分子快速导流杂交基因分型芯片（HybriMax）进行 HPV 基因分型，随机选4例HPV 58型阳性标本，进行基因克隆测序；进一步用 DNAstar ，clustalx 2．0和 Mega5．0软件对测序结果与 GenBank中发布的其他国家及国内的32株 HPV 58型病毒株 L1基因核苷酸和氨基酸序列建立同源性矩阵并分析，同时进行进化树构建。结果4株 HPV 58型病毒株之间的核苷酸同源性为96％～98％，氨基酸同源性为92％～98％；1986年法国株之间的核苷酸同源性为83％～86％，氨基酸同源性为84％～89％；1987年英国株之间的核苷酸同源性为53％～54％，氨基酸同源性为39％～42％；国内2010年香港分离株、2004年江苏分离株之间核苷酸的同源性为96％～100％，氨基酸同源性为92％～99％。从构建系统进化树图可以看出，4株本地区 HPV 58型病毒株分别与上海和香港 EV999963、DQ057326、HM639611病毒株基因背景最紧密。结论本地区 HPV 58型 L1基因具有一定的区域性特点。
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