目的：确定琼脂糖凝胶电泳中使用核酸染料Genefinder的最适方法，以期找到染色效果理想的染色方法。方法在琼脂糖凝胶电泳中，采用预染样品法、胶内染色法以及两者相结合的3种方法，使用核酸染料Genefinder进行染色，对染色结果进行比较分析。结果使用胶内染色法染色效果较好，预染样品法次之，胶染及样品染色法结合效果最差。结论核酸染料Genefinder会影响DNA的迁移效率，使用Genefinder进行胶内染色是一种较好的染色方法。%Objective To determine a optimal method for using nucleic acid dyes Genefinder in agarose gel e‐lectrophoresis in order to find out a staining method with ideal dyeing effect .Methods The pre‐dyeing sample meth‐od ,gel staining method and their combination staining method were adopted to perform the staining with nucleic acid dyes in agarose gel electrophoresis .The staining results were performed the comparative analysis .Results The Genefinder staining had better effect ,the pre‐dyeing sample method took the second place and the combination stai‐ning method had the worst effect .Conclusion Nucleic acid dyes Genefinder has an effect on the mobility of nucleic acid in agarose gel electrophoresis ,therefore staining nucleic acid with Genefinder is a better staining method in agar‐ose gel electrophoresis .
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