五色流式细胞术分类计数检测接尘工人外周血白细胞结果分析

摘要

Objective To explore the value of five-color flow cytometry leukocyte in detecting peripheral blood of underground pit miners,and to provide a new way to research the mechanism of lung injury caused by dust.Methods The levels of peripheral blood leukocytes subgroup were determined by a flow cytometer in 45 cases of healthy underground pit miners(dust-exposed group) and 40 cases healthy ground workers(control group).Results Compared with the control group,the levels of total monocytes,CD16-monocytes,CD16+ monocytes and cytotoxic T/NK cells were significantly higher in dust-exposed group(P<0.05),while the levels of other parameters were not significantly different(P>0.05).Conclusion The levels of total monocytes,CD16-monocytes,CD16+ monocytes and cytotoxic T/NK cells are higher in pit miners,which may be related to the early inflammatory of lung injury.%目的 探讨五色流式细胞术分类计数在接尘工人外周血白细胞中的检测价值,为粉尘导致肺损伤的机制研究提供新思路.方法 采用五色流式细胞术检测45例井下健康接尘矿工(接尘组)及40例井上健康人员(对照组)外周血白细胞亚群水平.结果 与对照组比较,接尘组总单核细胞、CD16-单核细胞、CD16+单核细胞、细胞毒性的T/NK细胞水平均增高,差异有统计学意义(P<0.05);其他指标间比较,差异无统计学意义(P>0.05).结论 煤矿接尘工人外周血总单核细胞、CD16-单核细胞、CD16+单核细胞、细胞毒性的T/NK细胞表达增加,可能与肺损伤早期炎症有关.