树突状细胞诱导的杀伤细胞治疗非霍奇金淋巴瘤患者的应用价值

摘要

Objective To explore the effect of DC‐CIK in the treatment of non Hodgkin lymphoma .Methods 84 cases of non Hodgkin′s lymphoma in Haikou People′s Hospital ,Central South University Xiangya Medical College Hospital from January 2011 to December 2014 were selected as the research object .The patients were randomly divided into observation group and control group by digital table method .The observation group was treated with DC‐CIK ,and the control group was treated with CIK .Will Ag‐DC centrifugal washing after the sensitization of CIK cells ,with 100 mL saline ,25 mL blood albumin rhIL‐2 and 200 000 unit weight suspension cells ,2 hours loss to the patient′s body back ,back to lose before giving intramuscular injection in patients with 25 mg promethazine hydrochloride .The therapeutic effect of the two groups was observed .Results Cell proliferation rate in observation group were significantly higher than the control group .And in the training on the sixth day ,number of cells in observation group were significantly greater than the control group .Cellular immune phenotypic differences of two groups of patients were compared , and the results showed that with the increase of incubation time ,DC ,CIK cells to CD3 + CD8 + and CD3 + CD56 + double the propor‐tion of positive cells were significantly higher than that of the same condition of CIK cells ,and DC‐CIK cells secrete factors levels were significantly higher than that of the same condition of CIK cells ,and the above differences are significant statistical difference (P< 0 .05) .Conclusion DC‐CIK cells have a very wide application prospect in the application of non Hodgkin lymphoma ,which is worthy of clinical application .%目的：探讨树突状细胞诱导的杀伤细胞（DC‐CIK）治疗非霍奇金淋巴瘤的临床效果。方法选取2011年1月至2014年12月该院收治的84例非霍奇金淋巴瘤作为研究对象，按数字表法将患者随机分为观察组与对照组，观察组患者采用 DC‐CIK 治疗，对照组患者使用细胞因子诱导的杀伤细胞（CIK）治疗。收集培养好且细菌检测为阴性的 DC‐CIK 或 CIK 细胞，应用无菌生理盐水清洗3次，将 Ag‐DC 致敏后的 CIK 细胞离心洗涤，用100 mL 生理盐水、25 mL 人血清蛋白和20万单位白细胞介素‐2（IL‐2）重悬细胞，2 h 内回输给患者，回输前给予患者肌注非那根25 mg 。观察并比较2组患者的临床治疗效果。结果观察组患者的细胞增殖速率显著高于对照组，培养第6天，细胞数明显大于对照组。随培养时间的增加，DC‐CIK 细胞 CD3＋／CD8＋、CD3＋／CD56＋的双阳性细胞比例显著高于同等条件下的 CIK 细胞，且 DC‐CIK 细胞的分泌因子水平显著高于同等条件下的 CIK细胞，差异均有统计学意义（P＜0．05）。结论 DC‐CIK 细胞治疗非霍奇金淋巴瘤具有明显的临床效果，值得进一步深入研究。