健康成年男性血栓弹力图参考值范围的研究

摘要

目的 测定健康成年男性血栓弹力图(T EG)的参考值范围,并与厂家提供的数据进行比较.方法 采集60例北京地区中国健康成年男性志愿者外周静脉血,应用西芬斯血栓弹力图仪LEPU-8800(北京乐普)分别测定凝血反应时间(R)、凝血形成时间(K)、凝固角(a-Angle)、30 min纤维蛋白溶解率(LY30)、综合凝血指数(CI)、二磷酸腺苷(ADP)诱导的血小板抑制率及M A 值.结果 60例志愿者平均年龄(24.84 ± 4.21)岁,TEG各参数范围分别为 R:4.89~10.77 min,K:1.31~4.05 min,a-Angle:42.64°~66.16°,MA:41.35~62.39 mm,LY30:―2.35% ~ 1.95%,CI:―7.12~ 0.32.4项主要指标(R、K、a-Angle、M A)中53.3%(32/60)的志愿者至少有1项指标在厂家参考值范围之外.根据Kaufmann分类法,20%(12/60)的志愿者被诊断为凝血异常.ADP抑制率检测37例小于20%,23例约为20% ~ <50%;M A值检测42例约为31~47 mm,18例大于47 mm.结论 北京地区健康成年男性 T EG参数实际参考值与厂家提供的参考值存在差异,实验室应对该地区人群特点建立新的 T EG参考值范围.%Objective To verify the reference interval for thromboelastography(TEG)in Chinese healthy a-dult males in Beijing and compare the value with those provided by the manufacturer.Methods A total of 60 peripheral vein blood samples from Chinese healthy adult males in Beijing were enrolled in this study.The T EG parameters including reaction time(R),coagulation time(K),angle(alpha),LY30,coagulation index (CI),ADP induced platelet inhibition rate and maximum amplitude(MA)were determined by using a LEPU-8800 device(LEPU,Beijing).The difference between the actual normal range and manufacturer′s provided reference range was compared.Results The average age of the recruited subjects was(24.84 ± 4.21)years old.T he measured ranges of the T EG parameters w ere R:4.89―10.77 min,K:1.31―4.05 min,alpha-an-gle:42.64―66.16,M A:41.35―62.39 mm,LY30:―2.35% ―1.95%,CI:―7.12―0.32.According to the values of the main indicators including R,K,alpha-angle,and M A,53.3%(32/60)volunteers had at least one indicator outside the manufacturer′s reference range.According to the Kaufmann classification,20%(12/60) volunteers were diagnosed with abnormal coagulation.The ADP inhibition rate was less than 20% in 37 cases, and between 20% ―<50% in 23 cases.The ADP(MA)values ranged between 31―47 mm in 42 cases and >47 mm 18 cases.Conclusion The actual range of TEG parameters for Chinese healthy adult males in Beijing is different from the reference interval provided by the manufacturers.It is necessary to establish the reference intervale of TEG parameters according to the characteristics of local population.