应用PCR方法快速鉴定SPF金定鸭性别

摘要

Objective To determine the sex of SPF Jinding Duck,the universal primer gCHD of avian chromosomal DNA-binding protein 1 (CHD1) gene on avian chromosomes were synthesized.Methods Blood of 85 SPF Jinding Duck were collected and genomic DNA was extracted for PCR amplification.The sex of the birds were determined by analyzing the number of bands after gel electrophoresis.Results The CHD 1-Z and CHD 1-W were found in the PCR products of agarose gel electrophoresis from females and CHD1-Z was from males.Conclusions The PCR can be used as an effective method for sex identification in birds.%目的 利用PCR方法鉴定SPF金定鸭的性别.方法 根据已有报道合成一对禽类性染色体上的染色质解螺旋蛋白DNA结合蛋白1(CHD1)基因的通用引物gCHD.采集培育中的85只SPF金定鸭血液样品,提取血液基因组DNA,进行PCR扩增.通过分析PCR扩增产物凝胶电泳后的条带数判定禽类性别.结果 PCR扩增产物经琼脂糖电泳分析后,雌性鸭样品扩增出CHD 1-Z和CHD1-W,而雄性鸭样品只出现CHD1-Z.结论 本文建立的方法可作为禽类性别的有效鉴定方法.