紫杉醇缓释微球对卵巢癌裸小鼠原位移植瘤的抑瘤效果

摘要

目的 研究以新型聚乳酸磷酸酯聚合物(PLCE)为载体的紫杉醇缓释微球体内抑瘤效果.方法 采用HO8910人卵巢癌细胞株,手术接种于68只BALB/c裸小鼠卵巢包膜下.3周后,取肿块大小相对均匀的42只裸小鼠随机分成7组:空白对照组、聚合物空白微球对照组、紫杉醇注射液组,紫杉醇缓释微球1中、低剂量组,紫杉醇缓释微球2中、低剂量组,每组6只.分组当日给药1次,连续观察一个月.结果 紫杉醇注射液抑瘤率57.0％;紫杉醇缓释微球2中剂量抑瘤率77.1％,低剂量抑瘤率58.7％.结论 以新型PLCE为载体的紫杉醇缓释微球2(60 mg/kg)对卵巢癌裸小鼠原位移植瘤有良好的抑制效果,但是毒性仍然较大,需要进一步改进.%Objective To observe the antitumor effect of paclitaxel-novel copolymer of poly lactic acid phosphate (PLCE) microspheres on proliferation of ovarian cancer.Methods The human overiam cancer (HO8910) were implanted into the ovarian capsule of 68 nude mice.Three weeks later,42 tumor beating nude mice were randomly divided into 7 groups as follow,blank control group,negative control group,paclitarel injection group,paclitaxel-PLCE microspheres 1 high and low dose group,and paclitaxel-PLCE microspheres 2 high and low dose group,with 6 nude mice in each group.The drug was administered 1 time a day,and the growth of the tumor were continuously observed for one month.Results The tumor inhibition rate of paclitarel injection was 57.0％,and tumor inhibition rate of paclitaxel-PLCE microspheres 2 high and low dose group were 77.1％ and 58.7％ respectively.Conclusion Paclitaxel-PLCE microspheres have a strong inhibitory effect on HO8910 ovarian cancer transplanted in nude mice.