HPLC法同时测定人血浆中帕罗西汀和喹硫平浓度

摘要

Objective To establish a method for determining paroxetine and quetiapine in human plasma by HPLC. Methods A reverse phase HPLC system was performed on Inertsil ODS-C18 column(4. 6 X 150 mm, 5μm) with the mobile phase consisted of acetonitrile-0. 05 mol/L NaH2PO4(39:61). The flow rate was 1.0 mL/min and the detection wavelength was at 210 nm. The column temperature was kept at 40℃. Ethylacetate and dichloromethane (75: 25) was used as extracting solvent. Results The calibration curves were linear in the range of 10 ~ 640μg/L for paroxetine, 20 ~ 1200μg/L for quetiapine respectively. The minimum detectable concentration for paroxetine and quetiapine were 5μg/L and 8μg/L respectively. Conclusion The method is simple, quick, sensitive and accurate, which can be used for clinical drug monitoring and pharmacokinetics studies of paroxetine and quetiapine.%目的 建立同时测定人血浆中帕罗西汀和喹硫平浓度的高效液相色谱( HPLC)法.方法 采用HPLC法进行测定:Inertsil ODS-C18柱(4.6×150mm,5μm)为色谱柱,流动相为乙腈-磷酸二氢钠(0.05 mol/L磷酸二氢钠)(39∶61),流速为1.0mL/m in,检测波长为210nm,柱温为40℃,以乙酸乙酯-二氯甲烷(体积比75∶25)为萃取剂.结果 帕罗西汀在10～640μ g/L、喹硫平在20 ～ 1200μg/L范围内峰面积与浓度呈良好线性关系,检测限分别为5μg/L,8μg/L.结论 该方法简单、快速、灵敏、准确,可用于临床帕罗西汀与喹硫平的血药浓度监测和药动学研究.