目的 探讨高血压患者血清8-异构前列腺素F2α(8-iso-PGF2α)及其氧化应激相关酶水平改变的临床意义.方法 选取193例原发性高血压患者(分为1、2、3级),应用双抗体夹心酶联免疫吸附法(ELISA)检测患者血清8-iso-PGF2α的水平；分别应用黄嘌呤氧化酶法和硫代巴比妥法进行超氧化物歧化酶(SOD)和丙二醛(MDA)的检测；将结果与52名正常健康人的上述指标比较.结果 原发性高血压1级、2级和3级患者的血清8-iso-PGF2α水平显著高于正常对照组(P＜0.05),并与血压水平呈正相关(r=0.62,P＜0.05)；原发性高血压1级、2级和3级患者的血清中SOD水平明显低于正常对照组(P＜0.01),随着血压的升高,SOD含量有下降的趋势,呈显著负相关(r=-0.74)；而MDA水平明显高于正常对照组(P＜0.01),随血压的升高,MDA含量有上升的趋势,呈显著正相关(r=0.81).结论 血清8-iso-PGF2α、SOD、MDA水平的变化与原发性高血压的发生和发展密切相关,临床检测上述指标有利于高血压病情的评估.%Objective To explore the changes of serum 8-iso-PGF2α, superoxide dismutase (SOD) and malondialdehyde( MDA) levels in patients with essential hypertension. Methods The content of 8-iso-PGF2α , SOD and MDA levels in 193 cases of essential hypertension and 52 controls were detected by using ELESA, xanthine oxidase and thiobarbituric acid method. Results The serum 8-iso-PGF2α levels in patients were obviously higher than those in controls ( P < 0. 05 ) , the SOD levels were significantly lower than those in controls ( P < 0.01) and the MDA levels were significantly higher than those in controls (P < 0.01). When the blood pressure increased, the content of SOD was decreased, and there was a significantly negative correlation (r = - 0. 74). But to the 8-iso-PGF2α and MDA, when the blood pressure increased the content of them was increased, There was a significantly positive correlation between the blood pressure with 8-iso-PGF2α and MDA levels respectively ( r = 0.62, r =0.81). Conclusion The levels of serum 8-iso-PGF2α , SOD and MDA are closely correlated to the severity of hypertension, and they may be used for evaluation of patients with hypertension.
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