Objective To develop a rapid and simple colloidal gold immunochromatographic ssay ( GICA) for the detection of Vibrio cholerae group 01. Methods A colloidal gold immunochromatography test based on double-antibody sandwich assay for detecting Vibrio cholerae group 01 was developed. Its sensitivity, specificity and stability were then evaluated. A total of 208 clinical specimens were determined with the prepared GICA, and the results were compared with those by bacterial culture. Results Typical detecting time was less than 10 minutes per sample. The positive samples produced a positive signal on immunochromatographic strip, whereas the negative one did not. The sensitivity of the test was 1 x 105 cfu/ml. Both the specificity and sensitivity of the prepared GICA in determination of 208 clinical specimens were 100%. Conclusion The gold-immunochromatography test appears to be a rapid, convenient, specific and sensitive test for detecting Vibrio cholerae group 01 on site.%目的 建立一种快速、简易的检测霍乱弧菌O1群的胶体金免疫层析法(GICA).方法 利用胶体金免疫层析技术,采用双抗体夹心法检测霍乱弧菌O1群,对该法进行敏感性、特异性和稳定性分析.与细菌培养法对比检测208份临床标本.结果 该法能在10分钟内完成检测；该试纸条仅与霍乱弧菌O1群阳性样品发生特异性反应；检测霍乱弧菌O1群的最低检出浓度为1×105cfu/mL；与细菌培养法对比检测208份临床标本,特异性和灵敏度均达100％.结论 新建立的霍乱弧菌O1群胶体金免疫层析试验简便、快速,特异性和灵敏度较好,适用于现场样品的快速筛查.
展开▼
