肺癌组织中HSP70-1+190(G/C)多态性对HSP70-1mRNA及HSP70蛋白表达的影响

摘要

Aim:To investigate the effect of heat shock protein(HSP) 70-1 gene polymorphism on the expressions of HSP70-1 mRNA and HSP70 protein. Methods: PCR-RFLP was applied to detect the single nucleotide polymorphisms (SNPs) of HSP70-1 + 190 (C/C) in 46 patients with lung cancer, RT-PCR was applied to measure the expression of HSP70-1 mRNA, and Western blot was applied to detect the level of HSP70. Results:The expression levels of HSP70-1 mRNA and protein in lung cancer tissue were obviously higher than those in the adjacent normal tissues (t = 19. 968, 17.794,P<0.001) , and they were related with the differentiation of lung cancer (t =6. 813 ,9. 386,P <0.001). A highly positive correlation existed between levels of HSP70-1 mRNA and HSP70 protein in lung concer tissue (r =0. 817,P < 0.001). Among the 46 cases,CC.GG and GC genotypes accounted for 23. 9% ,37.0% ,and 39. 1% .respectively. There were significant differences in the expression levels of HSP70-1 mRNA and HSP70 protein among the three genotypes ( F = 102.607,62.617, P <0.001 ). Compared with the other two genotypes, both the levels of HSP70-1 mRNA and HSP70 protein were apparently higher in the individuals with CC genotype(P <0. 001 ). Conclusion: CC genotype of HSP70-1 + 190 ( G/C) may be a high-risk factor for lung cancer and affect genetic transcription and protein expression.%目的：探讨热休克蛋白70(HSP70)-1+190(G/C)位点基因多态性对肺癌组织中HSP70-1 mRNA及HSP70蛋白表达的影响。方法：应用PCR-RFLP技术检测46例肺癌及相应癌旁正常组织中HSP70-1+190(G/C)位点多态性，RT-PCR法检测HSP70-1 mRNA水平，Western b1ot测定HSP70蛋白。结果：肺癌组织中HSP70-1 mRNA与HSP70蛋白的表达明显高于癌旁正常组织(t=19.968和17.794，P＜0.001)，并且其表达水平与肺癌的分化程度有关(t=6.813，9.386，P＜0.001)；肺癌组织中HSP70-1 mRNA与蛋白表达水平呈正相关(r=0.817，P＜0.001)。46例中，CC型11例(23.9％)，GG型17例(37.0％)，GC型18例(39.1％)，不同基因型携带者HSP70-1 mRNA和HSP70蛋白表达水平差异均有统计学意义(F=102.607和62.617，P＜0.001)，CC基因型携带者表达水平高于GG和GC基因型携带者(P＜0.001)。结论：HSP70-1+190(G/C)CC基因型可能是肺癌的一个高危险因素，并能影响其mRNA和蛋白的表达。