人抗黏液病毒A基因重组腺病毒体外抗乙型肝炎病毒的作用

摘要

目的:研究人抗黏液病毒A(MxA)基因重组腺病毒AdMxA体外抗乙型病毒性肝炎病毒(HBV)的作用效果.方法:以1倍感染复数(MOI)(1倍MOI感染组)及5倍MOI(5倍MOI感染组)的重组腺病毒AdMxA分别转染HepG2.2.15细胞,以未感染HepG2.2.15细胞为对照(未感染组).利用RT-PCR检测3组HepG2.2.15细胞内MxA mRNA水平表达,并检测3组HepG2.2.15细胞内HBsAg及HBeAg滴度变化.结果:3组HepG2.2.15细胞内MxA mRNA水平比较差异有统计学意义(F=226.294,P<0.001),3组细胞内HBsAg、HBeAg水平比较差异有统计学意义(F值分别为94.519和44.065,P均<0.001),而1 MOI及5 MOI感染组间差异无统计学意义(P>0.05).结论:重组腺病毒AdMxA感染HepG2.2.15后其MxA mRNA表达水平明显升高,并能显著抑制HepG2.2.15细胞中HBV的复制及表达,重组腺病毒AdMxA有望成为一种有效的基因治疗方法.%Aim: To study the relationship between the methylation and imprinting status of p73 gene promoter region and non-Hodgkin's lymphoma. Methods: A total of 40 lymph node biopsy specimens, including 20 specimens of nonHodgkin's lymphoma( 16 specimens of B-cell lymphoma,4 specimens of T-cell lymphoma) and 20 specimens of reactive hyperplasia of lymph node. We detected the gene imprinting and promoter region CpG Island methylation status of p73 gene in these tissues by reversed transcript PCR with restriction fragment length polymorphism(RT-PCR-RFLP) method and methylation-specific polymerase chain reaction(MS-PCR) method, respectively. Results:There were eight cases of heterozygous phenotype of p73 gene in 20 cases of non-Hodgkin' s lymphoma,in which the number of cases of p73 gene monoallelic phenotype was seven; There were six cases of heterozygous phenotype of p73 gene in 20 cases of reactive hyperplasia of lymph node,in which the number of cases of p73 gene monoallelic phenotype was five. p73 ailelotype between the two