Aim: To study the effects of zinc on expressions of Bcl-2 and Bax protein caused by fluoride in spermato-genic cells of male rats. Methods: A total of 50 Wistar male rats were randomly divided into control group, fluorine treatment group, fluorine and low-dose zinc treatment group, and fluorine and middle-dose zinc treatment group, fluorine and high-dose zinc treatment group. The content of NaF in testis was measured by using fluorine selective electrode. Changes of testosterone and expressions of Bcl-2, Bax protein in spermatogenic cells were respectively observed using the methods of ra-dioimmunoassay, immunohistochemical study. In addition,the quality of spermatozoa was observed. Results:The testosterone levels in serum of the three dose add zinc groups were higher than those of fluorine group and control group ( F = 129. 179, P < 0.001). Bcl-2 expression in spermatogenic cells of each zinc treatment groups was higher than that of fluorine treatment group(F =69.918 ,P < 0.001). Bax expression in spermatogenic cells and the testis fluoride content of mid-dose zinc treatment group were lower than those of fluorine treatment group( F = 52. 142 and 453. 812,P <0.001). Conclusion: Zinc could antagonize the reproductive toxicity of fluoride in male rats by increase Bcl-2 protein expression and decrease Bax protein expression in spermatogenic cells. Middle dose of zinc has the beet effect, high dose of zinc has no effect, even may has synergism with sodium fluoride.%目的:探讨锌对氟致雄性大鼠生精细胞Bcl-2和Bax蛋白表达的影响.方法:选用4～5周龄雄性Wistar大鼠50只,随机分为对照组、染氟组、氟加低锌组、氟加中锌组和氟加高锌组,灌胃染毒6周后处死动物,采用放射免疫法检测血清睾酮含量,免疫组织化学法检测生精细胞中Bcl-2和Bax蛋白的表达水平,氟离子选择电极法测定睾丸氟含量,并作精子质量分析.结果:氟加锌各剂量组血清睾酮和睾丸氟含量与对照组和染氟组比较,差异有统计学意义(F=129.179和453.812,P均＜0.001).氟加锌各剂量组Bcl-2和Bax蛋白表达水平与对照组和染氟组比较,差异有统计学意义(F=69.918和52.142,P均＜0.001).结论:锌可通过提高生精细胞Bcl-2蛋白表达、降低Bax蛋白的表达拮抗氟致大鼠生精细胞凋亡,中剂量锌拮抗作用较为明显,高剂量锌无拮抗作用甚至与氟产生协同作用.
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