Aim: To discuss the antagonism mechanism of zinc on reproductive toxicity of fluorine through observing the effects of appropriate amount zinc to the c-fos and c-jun protein expression level. Methods: Randomly divided fifty SD male rats into control group, fluorine treatment group, fluorine and low-dose zinc treatment group, fluorine and middle-dose zinc treatment group, fluorine and high-dose zinc treatment group. The content of NaF in testis was measured by using fluorine selective electrode. The levels of LDH were examined by the reagent box. The c-fos and c-jun protein expression level in germ cells are measured by immunohistochemistry. In addition, we observed the quality of spermatozoa and the micro-structure of testis. Results: The c-fos and c-jun protein expression in germ cells of every treatment groups was higher than that of control group,and the difference has the statistical significance,mid-dose zinc treatment group was lower than that of fluorine treatment groups, low-dose zinc and high-dose zinc treatment groups,and the difference has the statistical significance(F = 130. 251 ,P =0.031 ;F = 301. 141 , P = 0. 018 ). Conclusion: Middle-dose zinc can antagonize the reproductive toxicity of fluorine in male rats by lowering the fluorine burden, lowering the expression level of c-fos and c-jun protein expression in testis spermatogenic cell.%目的:观察锌对染氟大鼠生精细胞c-fos、c-jun蛋白表达水平的影响.方法:选用4周龄雄性SD大鼠50只,随机分为对照组、染氟组、氟加低锌组、氟加中锌组、氟加高锌组,灌胃染毒6周后处死动物,采用氟离子选择电极法测定各组大鼠睾丸组织中氟的含量,用试剂盒检测睾丸组织中的乳酸脱氢酶(LDH)活力,做精于质量分析及睾丸组织病理学镜检,免疫组化法检测生精细胞中c-fos、c-jun蛋白的表达水平.结果:氟加中锌组和氟加高锌组睾丸氟含量明显低于染氟组;氟加低锌组和氟加高锌组睾丸氟含量高于氟加中锌组(F=456.820,P＜0.001).氟加中锌组大鼠睾丸生精细胞c-fos、c-jun蛋白阳性细胞数低于染氟组、氟加低锌组和氟加高锌组(F=130.251,P=0.031;F=301.141,P=0.018).结论:中等剂量的锌通过减低氟负荷、增强乳酸脱氢酶活性、降低e-fos、c-jun蛋白表达,发挥对氟生殖损伤的拮抗作用.
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