miR-106 b对 HeLa细胞顺铂化疗敏感性的影响

摘要

目的：探讨miR-106 b对宫颈癌HeLa细胞顺铂化疗敏感性的影响。方法：采用实时定量PCR检测转染miR-106b抑制剂和类似物后HeLa细胞中miR-106b的表达，MTT法及流式细胞仪检测转染后HeLa细胞对顺铂的敏感性和凋亡，Western blot检测细胞中Twist1、PTEN、p-AKT（Ser473）蛋白的表达。结果：miR-106b抑制剂可减少HeLa细胞中miR-106b的表达；而miR-106b类似物可增加HeLa细胞中miR-106b的表达。转染miR-106b抑制剂后，细胞对顺铂的敏感性增强、凋亡及PTEN蛋白表达增加，Twist1、p-AKT表达降低（P＜0．05）；转染miR-106b类似物后，细胞对顺铂的敏感性减弱，凋亡及PTEN蛋白表达降低，Twist1、p-AKT表达增加（P＜0．05）。结论：miR-106 b可能通过调节Twist1、PTEN和p-AKT的表达影响HeLa细胞对顺铂耐药。%Aim:To explore the effects of miR-106 b on chemosensitivity of HeLa cells to cisplatin and its possible mechanism.Methods:miR-106b inhibitors and mimics were transiently transfected into HeLa cells , and the expression of miR-106b was detected by real-time PCR.Then, the drug sensitivity of cells to cisplatin cell apoptosis and the expression levels of Twist1, PTEN and p-AKT were detected by MTT assay , flow cytometry and Western blot .Results:The expres-sion of miR-106 b was significantly decreased after the miR-106 b inhibitors transfection , and increased after the mimics transfection in HeLa cells.Moreover, knockdown of miR-106b expression dramatically increased cell chemosensitivity and apoptosis effects induced by cisplatin and the expression of PTEN , but decreased the levels of Twist 1 and p-AKT in HeLa (P<0.05).On the contrary, overexpression of miR-106b significantly decreased the cell chemosensitivity and apoptosis effects induced by cisplatin and the expression of PTEN , but enhanced the levels of Twist1 and p-AKT(P<0.05).Con-clusion:Cisplatin chemosensitivity of HeLa cells may be modulated by miR-106 b through changing the expressions of Twist1, PTEN and p-AKT.