血复康组方诱导人红白血病细胞分化的研究

摘要

[Objective]To observe the effects of the active ingredients of XueFuKang(XFK) on leukemic cell-HEL differentiation. [Method] HEL cells were treated with the extractions of indigo, Kudzuvine Root, Zedoray Rhizome, the effective ingredients of XFK, respectively, the differentiation of HEL cells were revealed by flow cytometry marked with CDllb,CD14, while the expression of GPA, GATA1 was detected via RT-PCR.[Results] The expression of CDllb,CD14 was increased in a certain range of concentration with the extraction of indigo and Zedoray Rhizome ,and maximal expression level of the two antigens was observed at 50ug/ml for the extraction of Indigo, 20ug/ml for that of Zedoray Rhizome respectively. But this phenomenon was not observed in that of Kudzuvine Root. The mRNA level of GPA, GATA1, the cell differentiation markers of HEL cells, was upregulated at 50ug/ml for Indigo, 20ug/ml for Zedoray Rhizome using RT-PCR. [Conclusion] The extractions of indigo and Zedoray Rhizome can promote the HEL cells differentiation, which is conceived by the CD1lb,CD14 antigens expression on cell surface and GPA, GATA1 mRNA expressions in HEL cells.%[目标]本实验研究血复康的抗肿瘤有效成分,观察其诱导具有JAK2突变的人红白血病(human erythroleukemia,HEL)细胞株分化的影响.[方法]分别提取血复康组方青黛、莪术、葛根中的抗肿瘤有效成分,使其有效成分中靛玉红、莪术油及葛根总黄酮纯度＞80％,配制所需浓度的溶液.选择不同浓度的青黛提取物、莪术提取物、葛根提取物作用于HEL细胞,应用流式细胞术检测诱导细胞分化过程中细胞表面抗原CD1 1b、CD14的表达情况；RT-PCR在mRNA水平检测细胞分化标志GPA、GATA1基因表达变化.[结果]青黛提取物、莪术提取物能在一定浓度范围内增加细胞表面CD11b、CD14等表达,青黛50μg·mL-1、莪术20μ g·mL-1作用显著；葛根提取物无明显作用.青黛提取物50μg·mL-1、莪术提取物20μg·mL-1在mRNA水平均能上调HEL细胞分化分子标志GPA、GATA1的mRNA表达.[结论]血复康组方青黛、莪术提取物能有效诱导HEL细胞分化.