壮通饮对糖尿病肾病大鼠肾皮质CTGF、TGF-β1、PAI-1、FN细胞因子mRNA表达的影响

摘要

目的：实验性研究壮通饮对糖尿病肾病（DN）大鼠肾皮质结缔组织生长因子（CTGF）、转化生长因子（TGF－β1）、血浆纤溶酶原激活物抑制物（PAI－1）、纤维连接蛋白（FN）细胞因子 mRNA 表达的影响，并探讨壮通饮对 DN 大鼠肾脏的保护作用机制。方法采用单肾切除术合并腹腔注射链脲佐菌素（STZ）诱导制备 DN 大鼠模型，并随机分为空白对照组、假手术组、模型组、卡托普利西药组（1 g／kg）和壮通饮低（6．8 g／kg）、中（13．6 g／kg）、高（27．2 g／kg）剂量7个组。于实验给药治疗6周后，用荧光实时定量 RT－PCR 法检测大鼠肾皮质 CTGF、TGF－β1、PAI－1、FN 细胞因子mRNA 的表达。结果壮通饮可以降低 DN 大鼠肾皮质 CTGF、TGF－β1、PAI－1、FN 细胞因子 mRNA 的表达。结论壮通饮能改善肾皮质 CTGF、TGF－β1、PAI－1、FN 细胞因子 mRNA 的表达，以减轻肾脏病理改变，对 DN 大鼠的肾脏具有一定的保护作用。%Objective This article aims to laboratory study the effects of Chinese herbal Zhuangtongyin on mRNA expression of connective tissue growth factor (CTGF),transforming growth factor (TGF-β1),plas-minogen activator inhibitor 1 (PAI- 1),fibronectin (FN)cytokines in renal cortex of diabetic nephropathy (DN)rats,and to explore the mechanism of Zhuangtongyin in protecting the kidney of DN rats. Methods DN rat models were induced and prepared by mono-nephrectomy combined intraperitoneal injection Streptozo-tocin (STZ),and the rats were randomly divided into 7 groups:a blank control group,a sham operation group,a model group,a western medicine of Captopril group,(1 g/kg)and Zhuangtongyin low dose (6.8 g/kg),medium dose (13.6 g/kg)and high dose (27.2 g/kg)groups.Six weeks after drugs administration,a fluorescent quantitative real-time RT-PCR was used to measure the mRNA expression of CTGF,TGF-β1, PAI-1,FN cytokines in rats renal cortex. Results Zhuangtongyin could down- regulate mRNA expres-sions of CTGF,TGF-β1,PAI-1,FN cytokines in renal cortex of DN rats. Conclusion Zhuantgongyin can protect to a certain extent of DN rats kidney by improving he mRNA expression of CTGF,TGF-β1,PAI-1, FN cytokines in rats renal cortex for lessening the pathological changes of kidney.