FOXO3 a在三氧化二砷影响大肠癌细胞β-catenin表达中的作用

摘要

目的：探究三氧化二砷（ As 2O 3）对侵袭相关因子β-catenin表达的影响是否与FOXO3a有关。方法采用脂质体转染 siRNA 以及 LY294002药物改变大肠癌 SW620细胞中 FOXO3a 的表达， Western blot 检测FOXO3a、β-catenin 蛋白表达水平，RT-PCR 检测FOXO3a、β-catenin mRNA 表达水平。结果 As 2O 3增加SW620细胞中FOXO3a蛋白及mRNA的表达，同时降低β-catenin 蛋白及mRNA表达（P＜0．05）。 FOXO3a基因干扰后，β-catenin蛋白及mRNA的表达上调（ P＜0．05）。 LY294002药物处理后，β-catenin 表达下调（ P＜0．05）。结论 As 2O 3对人大肠癌SW620细胞中β-catenin 表达的抑制作用可能与其增强 FOXO3a 的活性有关。%O bjective To investigate whether FOXO3a is involved in the changed expression of β-catenin in color-ectal cancer SW620 cells after exposure to arsenic trioxide (As2O3).Methods The expression of FOXO3a was changed by FOXO3a specific siRNA and LY294002.Then, the protein and mRNA expressions of FOXO 3a and β-catenin in colorectal cancer SW620 cells were detected by western blot and RT -PCR, respectively.Results As2 O3 can stimulate the protein and mRNA expressions of FOXO3a in SW620 cells, while reducing the expression of β-catenin at protein and mRNA levels (P<0.05).After FOXO3a gene silencing, the protein and mRNA expressions of β-catenin were ob-viously enhanced (P<0.05).But LY294002 treatment could remarkably inhibit the expression of β-catenin at protein and mRNA levels .Conclusion The inhibitory effects of As 2 O3 onβ-catenin in colorectal cancer SW 620 cells may be associate with its ability to enhance the activity of FOXO 3a.