银杏内酯A对大鼠全脑缺血／再灌注损伤的影响及其机制研究﹡

摘要

Objective To investigate the effects of ginkgolide A on global cerebral ischemia /reperfusion in SD rats . Methods A rat model of global cerebral ischemia/reperfnsion was established through the four -vessel occlusion ( 4-VO) method.SD rats were randomly divided into a sham -operated group (Group sham), an ischemia/reperfusion group (Group I/R) and a treatment group (Group GA).Then, the effects of GA on the neurological deficit score (NDS), infarct area of the brain, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of each group were evaluated .Result Six hours after global cerebral/ischemia reperfusion , Group GA produced remarkably re-duced NDSs , infarct area of the brain and MDA content , in addition to enhanced SOD activity .Conclusion GA can significantly improve the neurological function of rats after global ischemia /reperfusion and produce protective effects on ischemia/reperfusion injury in rats .%目的：探讨银杏内酯A对全脑缺血／再灌注大鼠脑组织损伤的保护作用及其相关机制。方法采用四动脉结扎法构建大鼠全脑缺血／再灌注模型。将大鼠随机分为假手术组（ sham组）、缺血／再灌注组（ I／R组）和缺血／再灌注给药组（ GA组）。观察银杏内酯A对各组大鼠神经功能缺损评分（ NDS）、脑梗死面积、脑组织内超氧化物歧化酶（ SOD）活力及丙二醛（ MDA）含量的影响。结果全脑缺血／再灌注6 h后，银杏内酯A能明显降低大鼠NDS分值，减少脑梗死面积，降低脑组织内MDA含量，同时提高SOD活性。结论银杏内酯A能显著改善大鼠全脑缺血／再灌注后的脑神经功能，减轻缺血／再灌注对大鼠脑组织的损害，具有一定的脑保护作用。