目的：探讨新疆维吾尔族和汉族患者的幽门螺杆菌分离株 cagA、vacA 基因型与常见胃病的相关性。方法采用聚合酶链反应（PCR）方法检测92例胃病患者（慢性胃炎、萎缩性胃炎、胃溃疡和胃癌）的幽门螺杆菌vacA 和 cagA 基因型。结果维吾尔族与汉族胃病患者中幽门螺杆菌 cagA 基因检出率高，均为84．8％，但 cagA基因的分布无明显差异。在92株菌株中 vacA m2、s1a、m1b、s2的检出率分别为67．4％、61．9％、18．5％和4．3％。维、汉族胃病患者最常见的 vacA 基因型为 s1a、m2。维吾尔族胃癌患者 vacA m2基因型检出率高于汉族胃癌患者，而 vacA m1b 基因型检出率低于汉族患者。汉族胃溃疡患者 vacA m2基因型检出率显著高于胃癌患者；各胃病组 vacA m1b 基因型检出率从高到底依次为慢性胃炎、胃溃疡、胃癌，差异有统计学意义（P ＜0．05）。结论胃癌患者的 vacA 基因型分布可能与民族的不同而有区别，此结果可能为未来幽门螺杆菌的基因治疗提供一定的研究基础。%Objective To study the correlation between Helicobacter pylori isolates cagA and vacA geno-types and the common stomach trouble in Uyghur and Han nationalities in Xinjiang.Methods Using poly-merase chain reaction (PCR)to examine H .pylori isolates vacA and cagA status of 92 patients with dif-ferent clinical presentations (chronic gastritis,atrophic gastritis,gastric ulcer and gastric carcinoma).Re-sults The prevalence of cagA status was high between the two ethnics (84.8%),but the distribution of status had no significant difference.The detection rate of vacA m2,s1a,m1b and s2 were 67.4%,62%, 18.5% and 4.3% among 92 isolates,respectively.The most prevalent vacA genotype was s1a/m2 in pa-tients.The prevalence of vacA m2 in Han gastric carcinoma patients was significantly lower than Uyghur gastric carcinoma patients,but the prevalence of vacA m1b was higher than Uyghur.The vacA m2 geno-type in Chinese Han gastric ulcer patients was significantly higher than gastric carcinoma patients;the prevalence of vacA m1b from high to low was as follow:chronic gastritis group,gastric ulcer,gastric car-cinoma,the different had statistically significant (P <0.05).Conclusion Different nationalities had differ-ent distribution of cagA status among gastric carcinoma patients.It′s result can provide certain research foundation of gene therapy of Helieobacter pylori in the future.
展开▼
