肠炎相关肠癌小鼠模型及急性肠炎小鼠模型特征研究

摘要

目的 探讨肠炎相关肠癌小鼠模型及急性肠炎小鼠模型特征.方法 雄性C57BL/6小鼠(实验组)给予5％葡聚糖硫酸钠(Dextran sulfate sodium,DSS)连续饮用5d,5d后换为饮用蒸馏水至21d,构建急性肠炎小鼠模型,对照组21d内均饮用蒸馏水.肠炎相关肠癌小鼠模型的构建方法:雄性C57BL/6小鼠(实验组)腹腔注射氧化偶氮甲烷(Azoxymethane AOM)1次,1 w后予3％DSS连续饮用5d,随后12d饮用蒸馏水,DSS/蒸馏水循环共3次.对照组腹腔注射同体积生理盐水,饮用蒸馏水.采用HE染色观察病理学特征变化,RT-PCR法检测急性肠炎炎症因子表达特征.结果 急性肠炎模型发生炎症过程中小鼠体质量降低,相关炎症因子白细胞介素6(IL-6)、白细胞介素10(IL-10)、白细胞介素17(IL-17)表达变化趋势与肠黏膜结构变化过程相符,在炎症剧烈阶段(6～9 d)时表达均上调,而随着肠黏膜结构的自我修复,表达趋于正常.AOM/DSS肠炎相关肠癌模型可在3个DSS循环后形成肉眼可见腺瘤.结论 本研究为利用此模型进行后期的治疗炎症性肠病(IBD)药物筛查提供可靠数据支持.%Objective To investigate characters of acute colitis mice model and colitis-associated cancer (CAC) mice model.Methods Builded up acute colitis mice model by 5％ DSS (Dextran sulfate sodium) oral administration for 5 days on C57BL/6 (test group) mice with distilled water till to 21 days.And set up colitis-associated cancer mice model by intraperitoneal injection of AOM (Azoxymethane) once followed by three cycles (5 days/cycle) of 3％ DSS oral administration to develop CAC tumors.HE staining way was used to check the pathological characters and RT-PCR technique was to check characters of cytokines expressions.Results Weight loss occurred during DSS-colitis process,and a high production of IL-10,IL-6 and IL-17 were found on 6-9 d after the treatment,meanwhile the epithelial status was destroyed most seriously then started repair itself in the 17 days or 21 days.Adenocarcinoma were visible in gut under microscope in CAC mice model.Conclusion This investigation may be useful as a convenient tool to validate candidate therapeutic drugs for IBD.