豚鼠心脏表面神经节丛SP、CGRP及VIP 的免疫组织化学研究

摘要

Objective To investigate the distribution and the chemicalproperties of three predominant superfical ganglial plexus which are the right atrial ganglial plexus(RAGP),the dorsal atrial ganglial plexus(DAGP) and the ganglial plexus between aorta and pulmonary artery(A-PGP) in the heart.Methods The study was performed concering the neurons and fibers of SP,CGRP and VIP-immunoreactive(IR) substance in three predominant superfical ganglial plexus of the guinea pig heart by immunohistochemical ABC method and observed by light microscope.Results The SP-IR and CGRP-IR neurons could not be found in the above-mentioned ganglial plexus.But SP-IR fibers distributed sparsely,CGRP-IR fibers were more densely than SP-IR fibers,especially A-PGP.The population of VIP-IR neurons and fibers were most dense and interweaved ganglial plexus.Conclusion There were actually SP,CGRP and VIP in three predominant superfical ganglial plexus of the guinea pig heart and the three peptides could possibly modify the activities of myocardiocytes and vessels of heart directly.%目的 研究SP、CGRP及VIP在心脏表面3个主要神经节丛，右心房神经节丛(RAGP)、心房背侧神经节丛(DAGP)及主动脉与肺动脉间神经节丛(A-PGP)的化学性质和定位分布。方法 应用免疫组织化学ABC法，对豚鼠心脏表面3个主要神经节丛的神经元及神经纤维进行了SP、CGRP及VIP免疫组织化学染色及光镜观察。结果 在上述三个神经节丛内，未发现SP及CGRP免疫反应阳性神经元，但可见少量SP免疫反应阳性神经纤维分布，CGRP免疫反应阳性神经纤维数量较SP阳性纤维密集，尤其在A-PGP更为明显；VIP免疫反应阳性神经元和神经纤维分布最为密集，交织成神经节丛。结论 豚鼠心脏表面主要神经节丛内确实存在SP、CGRP及VIP，并且这3种神经肽可能直接参与心肌细胞和心脏血管活动的调控。