诸氏鲻虾虎鱼染色体组型分析

摘要

通过研究诸氏鲻虾虎鱼(Mugilogobius chulae)的染色体,分析了该鱼的细胞遗传学特征。取该鱼胚胎细胞,经秋水仙素浸泡、低渗处理、卡诺氏固定液固定后,用常规空气干燥法制作染色体标本,吉姆萨(Giemsa)染色后对获得的中期分裂相进行核型分析。研究结果表明,诸氏鲻虾虎鱼的染色体数目(2n)为44,核型为10m+10sm+4st+20t,染色体总臂数(NF)为64,未发现多倍体现象,也未发现异型性染色体和随体。%In this study, the karyotype of Mugilogobius chulae was firstly reported. The examined materials are metaphase of the early embryo cells prepared by the colchicine-hypotonic-air drying technique. The results showed that M. chulae has 2n=44 diploid chromosomes and the karyotype of 2n=10m+10sm+4st+20t with its fundamental arm number (NF)=64. Neither the polyploidy chromosomes nor the sex chromosomes were found, and there was no visible evidence of the satellites.