目的：本实验为研发一种新型促进骨腱愈合材料———柠檬酸钙复合材料，并在合适动物实验模型中探究材料对骨腱愈合的作用。方法实验动物选用成年日本大白兔48只，将其随机分为6组，均采用双侧兔趾长伸肌腱模型，实验侧（右侧）注射注射型海藻酸钠-明胶-柠檬酸钙复合材料，对照侧（左侧）注射等量海藻酸钠－明胶液体。术后2、4、6、8、10、12周取材，行标本组织学染色观察，采用Image-Pro Plus 8.0图像分析软件测定肌腱周围每1mm2新生骨面积，进行生物力学测试。结果术后6周内，组织学示骨腱界面中，实验侧Sharpey纤维、纤维软骨、钙化软骨出现明显早于对照侧，骨腱间间隙明显减小；2、4、6、8、10周及12周，每1mm2中新生骨面积实验组均高于对照组；术后6周内，实验侧骨腱愈合抗牵拉强度明显高于对照侧。结论可注射型海藻酸钠-明胶-柠檬酸钙对骨腱愈合具有明显的促进作用，这种作用主要发生在早期。%Objective To develop a new kind of injectable composite material-alginate-gelatin-calcium cit-rate to enhance bone-tendon healing and investigate the enhancement of tendon to bone by using the osteoinductive biomaterial in appropriate animal models. Methods Forty-eight adult Japanese white rabbits were selected and randomly divided into 6 groups.All animals received the long digital extensor tendon modeling on both sides.On the experimental (the right) side,injectable alginate-gelatin-calcium citrate was injected.On the control (the left) side,equal amount of alginate-gelatin liquid was injected.Materials were drawn 2,4,6,8,10 and 12 weeks respec-tively from the six groups and the histopathologic changes were observed by staining.The amount of new bone for-mation over the grafted tendon was measured by Imagepro-Plus 8.0 and biomechanical tests were conducted.All ex-perimental data were analyzed with SSPS16.0.Results Histologic observation showed that the Sharpey fiber,fibro-cartilage and calcified cartilage appeared earlier on the experimental side than on the control side.The bone-tendon gap on the experimental side reduced significantly.The area of new bone around the grafted tendon in the Calcium Citrate group at 2,4,6,8,10 and 12 weeks had significant difference.Mechanical examination showed that within 6 weeks after surgery,the pullout strength was higher on the experimental side than on the control side.Conclusion Injectable alginate-gelatin-calcium citrate can significantly enhance tendon-bone healing in early stage.
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