Objective To establish a mouse model highly expressing Bcl-2 protein for study of Bcl-2 function in vivo. Methods The plasmid containing human bcl-2 gene was constructed, which was transferred to fertilized eggs of C57BL/6J × CBA hybrid mice by microinjection; the fertilized eggs were implanted in the uterus of falsely pregnant female mice. The bcl-2 gene-carrying mice were the "Founder" mice, the "Founder" mice and C57BL/6J × CBA hybrid mice were continuously backcrossed, and the transgenic mice expressing Bcl-2 were obtained by screening with PCR and Western blotting. Results Five "Founder" mice were obtained; the strains were successfully established from #6 and #14 mice. After 7 generations of backcross breeding and screening by PCR and Western blot, 42 transgenic mice highly expressing bcl-2PCR were obtained. Conclusion The mouse model highly expressing Bcl-2 protein has been successfully established in this study.%目的 建立Bcl-2高表达的转基因小鼠品系,为在活体动物上研究bcl-2蛋白的功能提供动物模型.方法 构建含人Bcl-2基因的质粒;通过显微注射将该质粒转入C57BL/6J×CBA的杂交小鼠的受精卵,并植入假孕母鼠的子宫,PCR检测后携带人Bcl-2基因的小鼠即为“Founder”鼠;将“Founder”鼠与C57BL/6J×CBA杂交小鼠不断回交,经PCR和Western bilotting筛选后即可获得阳性小鼠.结果 共获得5只“Founder”鼠,其中#6、#14成功建系,连续回交繁殖7代后,PCR和Western筛选后获得bcl-2高表达阳性小鼠42只.结论 成功将人bcl-2基因转入小鼠基因组内,并稳定传代,建立Bcl-2高表达小鼠,为进一步研究Bcl-2蛋白提供了很好的动物模型.
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