目的：以简便、特异的方法获取目的基因片段用于体外构建重组体。方法：以引物互为模板的聚合酶链反应合成目的基因片段。该目的基因片段经限制性内切核酸酶水解后被连接至克隆载体，克隆后经筛选并电泳鉴定。结果：以聚合酶链反应合成了目的基因片段，并获得相应重组体。经鉴定，该重组体确含目的基因片段序列。结论：成功利用聚合酶链反应获得目的基因片段并获得相应重组体，以作为进一步研究的基础。%Objective: To obtain the foreign DNA to cloning is introduced in this paper. As foreign DNAs, human angiogenin gene fragments(hAngf) were obtained with PCR and cloning for research. Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning. The PCR products become into inserts after digested by restriction endonuclease and then they were inserted into pBluescript vectors to be cloned. Because of the different length of nucleotide sequences between containing the insert or not after treated by several restriction endonucleases, the positive clones and Ang - recombinants were screened and finally they were identified with electrophoresis. Results: The hAng inserts were obtained successfully by PCR and the electrophoretogram showed that the hAngf - pBluescripts wanted were obtained by the means properly adopted. Conclusion: Enough human angiogenin gene fragments (foreign DNAs) were synthesized by PCR and then they were inserted into vectors to be recombined in vitro. The studies of clinical applications and other areas about human angiogenin will be able to be carried out.
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