普洱茶对非酒精性脂肪肝大鼠肠道脂肪吸收及粘膜屏障的干预研究

摘要

In this study, the influence of Pu-erh tea on the absorption of long chain fatty acids in non-alcoholic fatty liver disease (NAFLD) rats was analyzed. Moreover, the possible interventional mechanism was also discussed. Thirty-six SD rats were randomly divided into three groups, normal group (NG), NAFLD group, and Pu-erh tea group (PTG), 12 rats for each group. All rats were killed after 8 weeks. Rat weight, liver index, serum lipid level, intestinal lipid absorption protein (CD36), tight junction proteins (Occludin, ZO-1), and TNF-α mRNA were measured. Compared with NAFLD group, rat weight and serum LDL-C in PTG were decreased by 16.12% and 42.59%, respectively. In addition, the expressions of CD36 and TNF-α mRNA in PTG were decreased, while occludin and ZO-1 mRNA expressions were increased. Our results suggest that Pu-erh tea can regulate the intestinal absorption of long chain fatty acids and the tight junction proteins in rats, and it is especially effective in prophylaxis against ‘the first attack’ in the early stage of NAFLD.%研究了普洱茶调控非酒精性脂肪肝大鼠肠道对长链脂肪酸的吸收，并探讨了其可能机制。将36只 SD大鼠随机分成正常对照组、脂肪肝模型组和普洱茶干预组，每组12只。实验第8周末处死所有大鼠，测定大鼠体质量、肝指数及血清血脂水平；采用定量 PCR 测定大鼠肠道黏膜脂质吸收相关蛋白细胞分化抗原36（Cluster of differentiation 36，CD36）、紧密连接相关蛋白如闭锁蛋白（Occludin）、紧密连接蛋白（Zonula occludens 1，ZO-1）和肿瘤坏死因子α（Tumor necrosis factor-α, TNF-α）基因表达水平。研究结果表明，与模型组相比，普洱茶组大鼠体质量和血清 LDL-C 水平分别下降了16.12%和42.59%；此外，普洱茶组大鼠小肠组织 CD36、TNF-α表达水平明显降低，而 Occludin、ZO-1表达水平明显升高。研究发现，普洱茶对 NAFLD大鼠肠道长链脂肪酸吸收及肠道紧密连接蛋白有一定程度的调控作用，尤其是对 NAFLD 早期发生发展中游离脂肪酸过度沉积的“第一次打击”有防治价值。